Overview:

Compared to Taq DNA Polymerase, High Fidelity DNA polymerase has better specificity, higher yield, and faster amplification performance (4 - 5 sec/kb). Its amplification error rate is 81-fold lower than that of Taq. Matched with an optimized buffer system, this master mix can be used for PCR amplification of templates such as genomic DNA, cDNA, plasmid DNA, dU-containing DNA, and crude samples. It has excellent compatibility with GC-rich primer/template systems. This master mix contains two types of monoclonal antibodies, inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. Amplification will generate blunt-ended products. A loading dye in the master mix allows the PCR products to directly be loaded for electrophoresis after the reaction. On a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, the dye front migrates at the same rate as a DNA fragment of about 400 base pairs (Blue band ~400bp).

Formulation:

This master mix contains DNA Polymerase, dNTPs, and an optimized buffer system with two types of monoclonal antibodies and a fluorescent dye in a proprietary formulation.