RNase H (Glycerol Free) is an endoribonuclease enzyme in a glycerol free storage buffer that catalyzes the cleavage of RNA in an RNA/DNA substrate via a hydrolytic mechanism. This enzyme is supplied with a 10X reaction buffer.
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RNH01-E311B |
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500 U
5000 U
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$ 400 |
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Overview:
RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA in DNA/RNA hybrid strands but does not hydrolyze phosphodiester bonds in single-stranded and double-stranded DNA or RNA. RNase H is specially optimized and formulated according to the requirements of freeze-dried reagents. It can be used in endoribonuclease H-dependent PCR amplification systems, such as qPCR, and LAMP. Applications include: removal of mRNA before second-strand cDNA synthesis, removal of poly(A) ends of mRNA in the presence of Oligo(dT), and cleavage of specific RNA sites.
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