Overview:

RNase inhibitor is a large (~50kDa) protein that forms extremely tight complexes with certain ribonucleases and may play an important role in regulating the lifetime of RNA in vivo (1). It has an unusually high cystine content and is thus sensitive to oxidation (2). Murine RNase inhibitor is a mouse sourced recombinant protein expressed in E. coli. Mouse sourced RNase inhibitor contains two fewer Cys residues than human RNase inhibitor and is thus less oxidation sensitive. It can inhibit the activity of RNase A, B or C in a non-competitive way to protect RNA from degradation. Murine RNase Inhibitor (glycerol free) has excellent thermal stability and storage stability. Due to its reduced oxidation sensitivity, it is highly suitable for experiments (e.g. qPCR assays) that are sensitive to reducing agents.