Overview:

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. It has a point mutation which leads to removal of RNase activity. Compared to other M-MLV mutants that only have the pol gene of M-MLV, M-MLV (H-) retains complete protein structure and polymerase activity. It can be used for synthesis of longer cDNA or preparation of cDNA library.

References:

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2. Kievits T, et al: NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection. J Virol Methods. 1991, 35: 273-286.
3. Sun QM, et al: Expression, purification of humanVasostatin120-180 in Escherichia and its antiangiogenic characterization. Protein Expr Purif. 2005, 31: 311-317.