Overview:

Super Mix for qPCR (+gDNA Wiper) consists of an upgraded reverse transcriptase (RT) in an optimized buffer. This kit further improves the efficiency of cDNA synthesis and is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need to design primers across introns. 5 × Super Mix RT contains all components required for the reverse transcription reaction and can be started rapidly after the addition of template RNA and RNase-free ddH2O. This product is suitable for reverse transcription of animal, plant and microbial RNA, and the reaction products are compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.