Super Fidelity Master Mix is a special next generation premix for robust PCR with higher fidelity. This product contains 2X master mix to which primers and template can be added. It has excellent long-fragment amplification ability, amplification specificity and amplification yield.
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SF01-E011 |
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1 ml
5 ml
15 ml
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$ 165 |
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Overview:
Super Fidelity DNA polymerase has a unique elongation factor, specificity enhancing factor, and plateau phase anti-inhibitor factor. These features greatly improve its long-fragment amplification ability, specificity, and PCR yield, compared to conventional polymerases like Taq DNA polymerase. Super Fidelity DNA polymerase is suitable for PCR amplification using genomic DNA, cDNA, Plasmid DNA and crude samples as templates. It can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. Its amplification error rate is 128-fold lower than that of conventional Taq DNA Polymerase. Additionally, Super Fidelity DNA polymerase has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples.
This master mix contains two types of monoclonal antibodies, inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. Protective agents in the mixture improve its resistance to repeated freezing and thawing. Amplification will generate blunt-ended products.
Formulation:
This master mix contains DNA Polymerase, dNTPs, and an optimized buffer system with two types of monoclonal antibodies in a proprietary formulation.
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