Overview:

Compared to Taq DNA Polymerase, Taq Plus DNA Polymerase has higher fidelity, stronger amplification performance, and higher yield. Taq Plus Master Mix II (Dye Plus) can be used for PCR amplification of animal, plant, and microbial DNA. The Master Mix can perform amplifications within 10 kb when genomic DNA is used as a template and within 15 kb when plasmid and λDNA is used as template. A protective agent in the buffer system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. The PCR product generated has A-tailing at the 3' end and can be cloned into T vectors. Loading dyes in the master mix allow the PCR products to be directly loaded for electrophoresis after the reaction. On a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, the dye fronts migrate at the same rate as DNA fragments of about 4000 and 500 base pairs each (Blue band 4000bp, Purple band 500bp).

Formulation:

This master mix contains Taq Plus DNA Polymerase, dNTPs, an optimized buffer system, and loading dyes in a proprietary formulation.