Bovine heat shock protein 70 (Hsp-70) ELISA Kit is for the quantitative determination of Hsp-70 levels in bovine serum, plasma, culture media, or any biological fluid.
BHS01-875
$ 545
96 Reactions Per Kit
Overview:
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Hsp-70. Samples are added to the microplate wells and bind the specific antibody. Then a biotinylated polyclonal antibody specific to TGF-β2 is added to each well and incubated forming an antibody-antigen complex. Following a wash to remove any unbound reagent, HRP-avidin is added to each well to bind Biotin-labeled antibody. After the unbound HRP-avidin has been washed away a color reagent is added to each well for coloration. Wells that contain Hsp-70 will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm.
Components:
No product specification information available.
No assay workflow and technical manual information available.
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