For all our products, we run an SDS-PAGE gel with a titration of the enzyme and perform densitometry measurements to determine purity.
An enzyme titration curve and calculated specific activity are included in the data sheet of each active enzyme. We recommend that researchers perform a serial dilution of an enzyme product to determine the optimal amount to use in their assay systems.
Our active enzymes are activated using specific protocols during the expression and production process to achieve optimal post-translational modification and in vitro activity. The treatment methods may vary for different enzymes, depending on their functional roles.
SignalChem Diagnostics carefully selects the expression system, either E.coli or Sf9 insect cells, based on the function and application of each product
SignalChem's active enzymes are validated for in vitro activity assays. We offer a wide range of custom services, including additional purification steps, buffer exchange, and tag modification, to fulfill the requirements for other applications such as protein crystallography. Please contact us for more information about this service.
For technical support on a specific product, please reach out to us by sending an email to info@signalchemdx.com. Our experts are committed to providing detailed information and guidance, ensuring the success of your experiments and applications.
