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2019-nCoV N-S1 Human IgA ELISA Kit


C19NS1-A877

$ 590

96 Reactions Per Kit





Overview:

The 2019-nCoV N-S1 Human IgA ELISA Kit is an enzyme-linked immunosorbent assay for the panspecific detection of Human anti-2019-nCoV N Protein & Spike Protein S1 IgA in serum or other biological samples. Based on indirect ELISA principles, the 2019-nCoV N-S1 Human IgA ELISA employs recombinant 2019-nCoV N Protein - Spike Protein S1 RBD Chimera coated on a 96- well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the target IgA in the controls and samples binds to the immobilized protein chimera. The intensity of color development in the wells is proportional to the amount of target IgA bound.


Components:

Part Number Part Size Storage
Kit BoxCP04-C19NSD2019-nCoV RBD-N Protein Chimera Coated Plate1 plate/1Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
WB20-09Wash Buffer, 20X15 ml/2
C19NS1A-POS-nCoV N-S1 IgA POS Control1 ml/1
C19NS1A-NEGnCoV N-S1 IgA NEG Control1 ml/1
SS01-09Stop Solution6 ml/1
E02-09nCoV Ig Sample Dilution Buffer10 ml/2
CS02-506One-Step TMB Substrate10 ml/1
H38A-63DGAnti-Human IgA Fc Detection Antibody, 1X10 ml/1

References:


1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.

4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.



Representative Data:

Calculation and interpretation of assay results

Representative Data

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Name Catalog Number Species Tag Expression System Sequence Genbank Number
2019-nCoV Spike protein RBD, HIS TagC19SD-G241H2019-nCoV (virus)HisCHO cells319-541MN908947
2019-nCoV Spike protein RBD, Fc TagC19SD-G241F2019-nCoV (virus)FcCHO cells319-541MN908947
2019-nCoV Spike protein S2, Fc TagC19S2-G241F2019-nCoV (virus)FcCHO cells686-1212MN908947
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated C19S1-60DH
Anti 2019-nCoV Spike Protein mIgG AntibodyC19S1-61MH
Anti 2019-nCoV Spike Protein hIgA AntibodyC19S1-A60H
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugatedC19NP-60DH
2019-nCoV S1 Protein ELISA KitC19SD-876N/A
2019-nCoV S1 Human IgG ELISA KitC19S1-877N/A
2019-nCoV S1 Human IgA ELISA KitC19S1-A877N/A
2019-nCoV S1 Human IgG-IgA-IgM ELISA KitC19S1-P877N/A
2019-nCoV N-S1 Human IgG-IgA-IgM ELISA KitC19NS1-P877N/AN/A


No assay workflow and technical manual information available.


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