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2019-nCoV S1 Human IgG-IgA-IgM ELISA Kit


C19S1-P877

$ 590

96 Reactions Per Kit





Overview:

The anti-nCoV S1 Human IgG-IgA-IgM ELISA Kit is an enzyme-linked immunosorbent assay for the pan-specific detection of Human anti-nCoV Spike protein S1 IgG-IgA-IgM in serum or other biological samples. Based on indirect ELISA principles, the anti-nCoV S1 Human IgG-IgA-IgM ELISA employs recombinant nCoV Spike protein RBD coated on a 96-well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the target IgG-IgA-IgM in the controls and samples binds to the immobilized Spike protein. The intensity of color development in the wells is proportional to the amount of target IgG-IgA-IgM bound.


Components:

Part Number Part Size Storage
Kit BoxWB20-09Wash Buffer, 20X15 ml/2Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
C19S1P-POSIgG-IgA-IgM Positive Control1 ml/1
C19S1P-NEGIgG-IgA-IgM Negative Control1 ml/1
SS01-09Stop Solution6 ml/1
E02-09nCoV Ig Sample Dilution Buffer10 ml/2
CS02-506One-Step TMB Substrate10 ml/1
CP03-C19SD2019-nCoV Spike Protein RBD Coated Plate1 plate/1
H38P-63DGAnti-Human IgG-IgA-IgM Fc Detection Antibody, 1X10 ml/1

References:


1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.

4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.



Representative Data:

Calculation and interpretation of assay results

Representative Data

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Name Catalog Number Species Tag Expression System Sequence Genbank Number
2019-nCoV Spike protein RBD, Fc TagC19SD-G241F2019-nCoV (virus)FcCHO cells319-541MN908947
2019-nCoV Spike protein RBD, HIS TagC19SD-G241H2019-nCoV (virus)HisCHO cells319-541MN908947
2019-nCoV Spike protein S1, Fc TagC19S1-G241F2019-nCoV (virus)FcCHO cells16-685MN908947
2019-nCoV Spike protein S1, HIS TagC19S1-G241H2019-nCoV (virus)HisCHO cells16-685MN908947
2019-nCoV Spike protein S2, Fc TagC19S2-G241F2019-nCoV (virus)FcCHO cells686-1212MN908947
Anti 2019-nCoV Spike Protein mIgG AntibodyC19S1-61MH
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated C19S1-60DH
Anti-Human IgG Antibody mIgM, Unconjugated H38-60M
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugatedC19NP-60DH
2019-nCoV S1 Protein ELISA KitC19SD-876N/A
2019-nCoV S1 Human IgG ELISA KitC19S1-877N/A
2019-nCoV S1 Human IgA ELISA KitC19S1-A877N/A
2019-nCoV N-S1 Human IgA ELISA KitC19NS1-A877N/AN/A
2019-nCoV N-S1 Human IgG ELISA KitC19NS1-877N/AN/A
2019-nCoV N-S1 Human IgG-IgA-IgM ELISA KitC19NS1-P877N/AN/A
2019-nCoV Neutralization ELISA KitC19SD-N887N/AN/A
2019-nCoV High Sensitivity S1 Protein ELISA KitC19S1-876N/AN/A


No assay workflow and technical manual information available.


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