C19SD-N887
$ 620
96 Reactions Per Kit
Overview:
The 2019-nCoV Neutralization ELISA Kit is an enzyme-linked immunosorbent assay for the detection of antibodies or other proteins (molecular) that can block the binding of SARS-CoV-2 S1 protein and human ACE2 in serum or other biological samples. Based on indirect ELISA principles, the 2019- nCoV Neutralization ELISA employs recombinant human ACE2 protein coated on a 96-well plate. Controls and samples (with necessary dilution) are mixed with 2019-nCOV S1 protein RBD-HRP and then pipetted into the wells; the target neutralizing antibodies or proteins in the controls and samples will partially or completely block binding of the S1 protein RBD-HRP to the immobilized ACE2 protein. After washing away the unbound 2019-nCOV S1 protein RBD-HRP, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.
Components:
| Part Number | Part | Size | Storage | |
| Kit Box | C19SD-G241DH | 2019-nCoV Spike protein RBD, HRP Conjugate | 8 ml/1 | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
| CP02-A51C2 | ACE2 (19-740) Coated Plate | 1 plate/1 | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. | |
| E01-09 | nCoV ELISA Sample Dilution Buffer | 10 ml/1 | ||
| SS01-09 | Stop Solution | 6 ml/1 | ||
| C19N-NEG | NAb Negative Control | 150 ul/1 | ||
| C19N-POS | NAb Positive Control | 150 ul/1 | ||
| WB20-09 | Wash Buffer, 20X | 15 ml/1 | ||
| CS02-506 | One-Step TMB Substrate | 10 ml/1 |
References:
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.
Representative Data:
In order to evaluate the presence of Neutralizing antibodies or proteins in a biological sample, the O.D. value of the sample must be compared to the values obtained for the positive and negative controls.
Related Products:
| Name | Catalog Number | Species | Tag | Expression System | Sequence | Genbank Number |
|---|---|---|---|---|---|---|
| 2019-nCoV Spike protein RBD, HIS Tag | C19SD-G241H | 2019-nCoV (virus) | His | CHO cells | 319-541 | MN908947 |
| 2019-nCoV Spike protein RBD, Fc Tag | C19SD-G241F | 2019-nCoV (virus) | Fc | CHO cells | 319-541 | MN908947 |
| 2019-nCoV Spike protein S1, HIS Tag | C19S1-G241H | 2019-nCoV (virus) | His | CHO cells | 16-685 | MN908947 |
| 2019-nCoV Spike protein S1, Fc Tag | C19S1-G241F | 2019-nCoV (virus) | Fc | CHO cells | 16-685 | MN908947 |
| 2019-nCoV Spike protein S2, Fc Tag | C19S2-G241F | 2019-nCoV (virus) | Fc | CHO cells | 686-1212 | MN908947 |
| Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated | C19S1-60DH | |||||
| Anti 2019-nCoV Spike Protein mIgG Antibody | C19S1-61MH | |||||
| Anti 2019-nCoV Spike Protein hIgA Antibody | C19S1-A60H | |||||
| Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugated | C19NP-60DH | |||||
| 2019-nCoV S1 Protein ELISA Kit | C19SD-876 | N/A | ||||
| 2019-nCoV S1 Human IgG-IgA-IgM ELISA Kit | C19S1-P877 | N/A | ||||
| 2019-nCoV High Sensitivity S1 Protein ELISA Kit | C19S1-876 | N/A | N/A |
No assay workflow and technical manual information available.
There are no related publications available for this product.
2019-nCoV Spike protein RBD, Fc Tag, C19SD-G241F