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2019-nCoV Neutralization ELISA Kit


96 Reactions Per Kit


The 2019-nCoV Neutralization ELISA Kit is an enzyme-linked immunosorbent assay for the detection of antibodies or other proteins (molecular) that can block the binding of SARS-CoV-2 S1 protein and human ACE2 in serum or other biological samples. Based on indirect ELISA principles, the 2019- nCoV Neutralization ELISA employs recombinant human ACE2 protein coated on a 96-well plate. Controls and samples (with necessary dilution) are mixed with 2019-nCOV S1 protein RBD-HRP and then pipetted into the wells; the target neutralizing antibodies or proteins in the controls and samples will partially or completely block binding of the S1 protein RBD-HRP to the immobilized ACE2 protein. After washing away the unbound 2019-nCOV S1 protein RBD-HRP, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.


Part Number Part Size Storage
Kit BoxC19SD-G241DH2019-nCoV Spike protein RBD, HRP Conjugate8 ml/1Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
CP02-A51C2ACE2 (19-740) Coated Plate1 plate/1Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
E01-09nCoV ELISA Sample Dilution Buffer10 ml/1
SS01-09Stop Solution6 ml/1
C19N-NEGNAb Negative Control150 ul/1
C19N-POSNAb Positive Control150 ul/1
WB20-09Wash Buffer, 20X15 ml/1
CS02-506One-Step TMB Substrate10 ml/1


1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.

4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.

Representative Data:

In order to evaluate the presence of Neutralizing antibodies or proteins in a biological sample, the O.D. value of the sample must be compared to the values obtained for the positive and negative controls.

Related Products:

Name Catalog Number Species Tag Expression System Sequence Genbank Number
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No assay workflow and technical manual information available.

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