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2019-nCoV 3CLpro, Active

Recombinant full-length 2019-nCoV 3CLpro, part of a large replicase polyprotein 1ab (S3264-Q3569), was expressed in E. coli cells with an N-terminal His tag.


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$ 160


SARS-CoV-2 3C-like proteinase (3CLpro) is the nonstructural protein number 5, also a cysteine protease, that is released from the virally encoded polyprotein by extensive proteolytic processing. The 3CLpro cleaves the C-terminus of the large replicase polyprotein 1ab (~790 kDa) at no fewer than 11 sites (1). Inhibiting the activity of this enzyme would block viral replication (2). The functional importance of 3CLpro in the viral life cycle makes this protease an attractive target for the development of drugs directed against SARS and other coronavirus infections (3).

Gene Aliases:

SARS-CoV-2 3CLpro; NSP5; Mpro

Genebank Number:


50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1 mM DTT, 10% glycerol


1. Hegyi, A. et al: Conservation of substrate specificities among coronavirus main proteases. J Gen Virol. 2002,83:595-599.

2. Zhang L. et al: Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors. Science. 2020, 368:409-412.

3. Anand K. et al: Coronavirus main proteinase (3CLpro) structure: Basis for design of anti-SARS drugs. Science. 2003, 300:1763-1767.

There are no related publications available for this product.


Acute Respiratory Distress Syndrome , Cardiovascular Disease, Cell Cycle, Cellular Stress, COVID19, Cytoplasmic Tyrosine Kinases, Gastrointestinal Diseases , Infectious Diseases , Inflammation, Lung Diseases , Neurobiology, severe acute respiratory syndrome coronavirus 2 , Virology