Overview:

SARS-CoV-2 3C-like proteinase (3CLpro) is the nonstructural protein number 5, also a cysteine protease that is released from the virally encoded polyprotein by extensive proteolytic processing. The 3CLpro cleaves the C-terminus of the large replicase polyprotein 1ab (~790 kDa) at no fewer than 11 sites (1). Inhibiting the activity of this enzyme would block viral replication (2). The functional importance of 3CLpro in the viral life cycle makes this protease an attractive target for the development of drugs directed against SARS and other coronavirus infections (3).

Gene Aliases:

SARS-CoV-2 3CLpro; NSP5; Mpro

Genebank Number:

QHD43415

Formulation:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1 mM DTT, 10% glycerol.

References:

1. Hegyi, A. et al: Conservation of substrate specificities among coronavirus main proteases. J Gen Virol. 2002,83:595-599. 2. Zhang L. et al: Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors. Science. 2020, 368:409-412. 3. Anand K. et al: Coronavirus main proteinase (3CLpro) structure: Basis for design of anti-SARS drugs. Science. 2003, 300:1763-1767.

Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity:

Sample activity data. for lot-specific data, please refer to the respective data.