Recombinant tag-free Endonuclease (22-266) from Serratia marcescens was expressed in E. coli.
EN01-E311U |
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10000 U
25000 U
50000 U
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$ 105 |
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Overview:
Serratia marcescens endonuclease is a nonspecific nuclease with little sequence selectivity that can cleave single stranded, double stranded, linear, circular, or supercoiled DNA and RNA substrates (1, 2). The enzyme completely digests nucleic acids to 5’-monophosphate terminated oligonucleotides 2-5 bases in length and is effective over a wide range of operating conditions. It is extracellularly secreted by the Serratia marcescens bacteria and is thought to contribute to its virulence by scavenging nucleic acid from the extracellular environment (3). The enzyme may be used for various biotechnological applications including removing nucleic acids from protein samples, prevention of cell clumping, and replacement of sonication or mechanical shearing of DNA.
Genebank Number:
Formulation:
Recombinant protein stored in 50mM Tris (pH 8.0), 2mM MgCl2, 20mM NaCl, and 50% Glycerol.
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Cancer, Cell Cycle