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Malate dehydrogenase

Malate dehydrogenase is a diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of L-malate and of glutamate oxaloacetate transaminase (GOT) in clinical analysis.

MD01D1-E311H
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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

40U/mg-solid or more


Unit Definition:

One unit causes the oxidation of one micromole of NADH per minute at pH 7.5 and 30°C


Grade:

Diagnostic Reagent


EC No.:

EC 1.1.1.37


Appearance:

White amorphous powder, lyophilized


Contaminants:

Glutamate oxaloacetate transaminase ≤1.0 × 10-3 %
Lactate dehydrogenase ≤1.0 × 10-3 %
NADH oxidase ≤1.0 × 10-3 %


Molecular Weight:

33.4 kDa


Isoelectric Point:

6.25


Inhibitors:

Ag+, Hg2+, IAA


Optimum pH:

7.5


Optimum Temperature:

50°C


pH Stability:

pH 5.8–10.5 (25°C, 20 hr)


Thermal Stability:

Below 50°C (pH 7.5, 15 min)




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