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Sau DNA Polymerase Large Fragment

Recombinant tag-free Sau DNA polymerase large fragment from Staphylococcus aureus was expressed in E. coli cells.


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DNA polymerase (DNAP) is an enzyme that catalyzes the DNA molecules and is crucial for DNA replication. Sau DNA polymerase large fragment is a product of the Staphylococcus aureus DNA polymerase I that retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ and 3´→ 5´ exonuclease activity (1). Sau DNA polymerase has a wide range of applications such as primer labelling, second-strand cDNA synthesis, DNA strand displacement synthesis, recombinase polymerase amplification, and single dA tailing (2).

Gene Aliases:

polA, DNA polymerase I

Genebank Number:


Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 20% glycerol, 1 mM DTT.


1. Okazaki, T. et al. J. Biol.Chem. 1964, 239: 259-268. 2. Piepenburg, O. et al. PLOSBiology. 2006, 4: 1115-1121.

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