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2019-nCoV High Sensitivity S1 Protein ELISA Kit


96 Reactions Per Kit


The 2019-nCoV High Sensitivity S1 Protein ELISA Kit v2.0 is a sandwich enzyme-linked immunosorbent assay for the quantitative measurement of 2019-nCoV Coronavirus Spike protein. The S1 Protein ELISA employs a capture anti-S1 protein antibody coated on a 96-well plate. Calibrators and samples are pipetted into the wells; the target protein in the calibrators and samples binds to the immobilized capture antibody. The wells are washed and the HRP-labeled Detection antibody is then added. After washing away the unbound HRP-antibodies, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.


Part Number Part Size Storage
Kit BoxC19S1-HS61DHAnti-nCoV S1 Detection Antibody, HS-1X10 ml/1Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
CP06-642HLAnti 2019-nCoV Spike Protein Coated Plate1 plate/1
CS02-506One-Step TMB Substrate10 ml/1
E01-09nCoV ELISA Sample Dilution Buffer10 ml/2
SS01-09Stop Solution6 ml/1
C19SD-CAL50Calibrator 501 ml/1
WB20-09Wash Buffer, 20X15 ml/2


1.Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Lan J, et al: Crystal structure of the 2019-nCoV Spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.

Representative Data:

The 2019-nCoV Spike protein concentration of a biological sample may be calculated using the Calibrator by generating a standard curve that correlates the measured O.D. values.

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No assay workflow and technical manual information available.

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