Home   >    COVID-19   >   Covid-19 Proteins

Signalchem Product Image

2019-nCoV NSP7 Protein

Recombinant full-length viral NSP7 was expressed in E. coli using a C-terminal His tag.

C19N7-E541H

50 ug Bulk Customize

$ 360

*The image is illustrative and does not necessarily represent the product


Overview:

Novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). Coronavirus RNA genome encodes a complex replication machinery consisting of 16 viral non-structural proteins (NSPs). NSP7 and NSP8 stabilize regions of NSP12 (RNA-dependent RNA polymerase; RdRp) involved in RNA binding and are essential for a highly active NSP12 polymerase complex. NSP7 has been suggested to confer RNA-binding properties to the trimeric polymerase complex (2). NSP8 has been shown to act as an oligo(U)-templated polyadenylyltransferase and a robust (mono/oligo) adenylate transferase (3). The NSP7/NSP8/NSP12 polymerase complex associates with NSP14 which is required to safeguard coronavirus replication fidelity (2).


Gene Aliases:

NSP7: Non-structural protein 7


Genebank Number:


Formulation:

Recombinant protein stored in 20 mM Tris, pH 7.5, 300mM NaCl, 200mM imidazole, 1 mM DTT, 10% glycerol.


References:


1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Subissi L, et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. PNAS. U.S.A. 2014, 111: E3900–E3909.

3. Tvarogova J, et al: Identification and characterization of a human coronavirus 229E nonstructural protein 8-associated RNA 3'-terminal adenylyltransferase activity. Virol. 2019, 368: 779-782.




There are no related publications available for this product.


RESEARCH AREAS

Acute Respiratory Distress Syndrome , Cardiovascular Disease, Cell Cycle, Cellular Stress, COVID19, Gastrointestinal Diseases , Infectious Diseases , Inflammation, Lung Diseases , Neurobiology, severe acute respiratory syndrome coronavirus 2 , Virology



RELATED PRODUCTS