Overview:

β-thionicotinamide adenine dinucleotide (β-Thio-NAD) is an analog of the coenzyme nicotinamide adenine dinucleotide (NAD) (1). β-Thio-NAD is reduced to β thio-NADH. β-Thio-NAD It is used for various clinical chemistry applications including determining the levels of L-cartenine in serum by an enzymatic cycling method (2), ultrasensitive sandwich enzyme-linked immunosorbent assay (ELISA) assays for detection of infectious disease (3).

References:

1. National Center for Biotechnology Information (2022). PubChem Compound Summary for CID 3081386, Thio-NAD. Retrieved October 24, 2022 from https://pubchem.ncbi.nlm.nih.gov/compound/Thio-NAD.

2. M Takahashi, S Ueda, H Misaki, N Sugiyama, K Matsumoto, N Matsuo, S Murao, Carnitine determination by an enzymatic cycling method with carnitine dehydrogenase, Clinical Chemistry, Volume 40, Issue 5, 1 May 1994, Pages 817- 821, https://doi.org/10.1093/clinchem/40.5.817

3. Iha K, Inada M, Kawada N, Nakaishi K, Watabe S, Tan YH, Shen C, Ke LY, Yoshimura T, Ito E. Ultrasensitive ELISA Developed for Diagnosis. Diagnostics (Basel). 2019 Jul 18;9(3):78. doi: 10.3390/diagnostics9030078. PMID: 31323782; PMCID: PMC6787603.