Overview:

Ribonuclease H II (RNase H II) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA on RNA/DNA hybrid strands but does not hydrolyze phosphodiester bonds in single/double stranded DNA or RNA. The enzyme works by breaking the phosphodiester linking RNA bases in 5' direction to DNA bases, generating a 3' hydroxyl end and a phosphate group at the 5' end of the ribonucleic acid. RNase H II (Glycerol Free) is in a glycerol free storage buffer and is specially optimized and formulated according to requirements of freeze-dried reagents. It can be used in PCR amplification systems, qPCR, LAMP and other reaction systems that rely on endoribonuclease H.