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T4 gp32 Protein

Recombinant full-length T4 gp32 Protein from Escherichia virus T4 was expressed in E. coli using N-terminal His tag.

T4G32-C501H

50 ug 100 ug 200 ug 500 ug

$ 110

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Overview:

Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA binding protein with key role in DNA replication, recombination, and repair. T4 gp32 is a zinc metalloprotein with three functional domains: the N-terminal basic domain, the core domain and the C-terminal acidic domain. The ssDNA binding site lies in the core domain. In vitro, T4 gp32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase (1,2)


Gene Aliases:

ss-DNA binding protein (g32), SSB protein, Helix-destabilizing protein


Genebank Number:


Formulation:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 20% glycerol, 1 mM DTT.


References:

1. Jordan, C.S. et al: Regulation of the bacteriophage T4 Dda helicase by Gp32 single-stranded DNA-binding protein. DNA Repair (Amst), 2015; 25: 41-53. 2. Pant, K. et al: The role of the C-domain of bacteriophage T4 gene 32 protein in ssDNA binding and dsDNA helix-destabilization: Kinetic, single-molecule, and cross-linking studies. PLoS One. 2018; 10:13(4):e0194357.




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