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Taq DNA polymerase

Purified Thermus aquaticus DNA polymerase with 5'→3' polymerase activity and 5'→ 3' exonuclease activity, but no 3' → 5' exonuclease activity


1000 U/mL

$ 305


Taq DNA polymerase is a highly thermostable DNA polymerase of Thermus aquaticus. The enzyme exhibits 5'→3' polymerase activity and possesses 5'→3' exonuclease activity but has no detectable 3'→5' exonuclease activity. In addition, Taq DNA polymerase generates PCR products containing extra adenines at the 3'-ends. The resulting products can be used for TA cloning. Applications of this product include: routine PCR amplification of DNA fragments, generation of PCR products for TA cloning, DNA labelling and DNA sequencing.


1. Innis M A, et al: PCR protocols and applications: A laboratory manual, academic, New York, 1989.
2. Innis M A, et al: DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA, Proc. Natl. Acad. Sci. USA. 1988, 85:9436-9440.
3. Weyant R S, et al: Effect of ionic and nonionic detergents on the Taq polymerase. Biotechniques. 1990, 9:308-309.

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