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Recombinant full length, tag-free Alicyclobacillus acidiphilus AapCas12b protein expressed in E. coli. CRISPR enzyme sold with 1ml of Cas12 reaction buffer, 10x (CS12-09).
C12B1-E311U |
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50 ug
100 ug
Bulk Customize
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$ 120 |
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Overview:
CRISPR (clustered regularly interspaced short palindromic repeats) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been leveraged to create an exemplary genome editing tool with applications in both basic research and therapeutics development (3). AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation (4).
Accession Number:
Formulation:
Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 1mM DTT, 10% glycerol.
References:
1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4.Teng F, et al. 2018. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell discovery. 4(1):1-5.
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles
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