Overview:

CRISPR (clustered regularly interspaced short palindromic repeats) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been leveraged to create an exemplary genome editing tool with applications in both basic research and therapeutics development (3). AsCas12a is a Cas endonuclease from Acidaminococcus sp. Engineered AsCas12a systems can be used for accurate editing in hematopoietic stem cells, induced pluripotent stem (iPS) cells, and natural killer (NK) cells (2-4).

Accession Number:

U2UMQ6

Formulation:

Recombinant protein stored in 50 mM Tris pH 7.5, 600 mM NaCl, 10% glycerol, 2 mM DTT.

References:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.

2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The disconvery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.

3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.

4. DeLong, R. 2021. Ushering in a new era of RNA-based therapies. Commun Biol. 4(1):557.