AsCas12a

Alternate Names:

Cpf1, AsCpf1, CRISPR-associated endonuclease Cpf1

UniProt Number:

U2UMQ6

Overview:

CRISPR (clustered regularly interspaced short palindromic repeats) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been leveraged to create an exemplary genome editing tool with applications in both basic research and therapeutics development (3). AsCas12a is a Cas endonuclease from Acidaminococcus sp. Engineered AsCas12a systems can be used for accurate editing in hematopoietic stem cells, induced pluripotent stem (iPS) cells, and natural killer (NK) cells (2-4).

Accession Number:

WP_021736722.1

Formulation:

Protein stored in 25 mM Tris, 300 mM NaCl, pH 7.5, 0.1 mM TCEP, 50% glycerol

References:

1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. DeLong, R. 2021. Ushering in a new era of RNA-based therapies. Commun Biol. 4(1):557.

Storage and Stability:

Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.

Concentration:

1.0 μg/μl
6.67 pmol/μg

Purity:

>90%

Molecular Weight:

Calculated: 152.5 kDa
Observed: ~130 kDa

Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.