Home > CRISPR > CRISPR Enzymes
Recombinant tag-free full-length Leptotrichia buccalis LbuCas13a expressed in E. coli. CRISPR enzyme sold with 1ml of Cas13 reaction buffer II, 10x (CS13-09B).
CS13A-E322U |
|
50 ug
100 ug
Bulk Customize
|
|
$ 90 |
|
*The image is illustrative and does not necessarily represent the product
Overview:
Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells (4).
Accession Number:
Formulation:
Recombinant protein stored in 50 mM Tris-HCL, pH 7.5, 600 mM NaCl, 2 mM DTT, 10% glycerol.
References:
1. East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270–3.
2. Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573.
3. Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
4. Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.
There are no related publications available for this product.