Overview:

TtCsm6 is a dimeric RNA endonuclease associated with type III CRISPR-Cas systems (1). Like other Csm6 family proteins, TtCsm6 consists of a CARF (CRISPR associated Rossman fold) domain which binds cyclic oligoadenylate (cOA) and a HEPN (Higher Eukaryotes and Prokaryotes Nucleotide binding) domain which possesses ribonuclease activity (2). Upon activation, by the cyclic oligoadenylate the Csm6 HEPN active site degrades invasive RNA non-specifically. This property of the enzyme has been leveraged for the development of sensitive and robust nucleic acid detection tools with applications in both basic research and therapeutics development (4).

Accession Number:

Q53W17

Formulation:

Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

References:

1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. Rna 22(3):318-329.

2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.

3. Liu, T.Y., et al. Accelerated RNA detection using tandem CRISPR nucleases. Nat Chem Biol 17, 982–988 (2021).