Overview:

RecQ helicases catalyze double-stranded DNA unwinding in the 3’ to 5’ direction, which is driven by ATPase activity that is dependent on a bivalent cation (1). RecQ helicases are important in coordinating genome maintenance and preserving genomic integrity in prokaryotes and eukaryotes (2). E. coli RecQ plays an important role in resuming DNA replication at stalled replication forks after repairing UV-induced DNA damage (3). They are also important in preventing future chromosomal abnormalities that result from DNA damage (3).

Gene Aliases:

RecQ

Genebank Number:

WP_032255548.

Formulation:

Recombinant protein stored in 20mM Tris-HCl, 300mM KCl, 10% glycerol, 1mM DTT pH 8.0.

References:

1. Sharma, S. et al: Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability. Biochem J, 2006; 398(3): 319-337. 2. Bennet, R. J., Keck, J. L.: Structure and function of RecQ DNA helicases. Crit Rev Biochem Mol Biol., 2004; 39(2): 79-97. 3. Hishida, T. et al: Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev., 2004; 18(15): 1886-1897.

Storage and Stability:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.